《植物生理学报》 2016, 52(6): 967-974
通信作者:张利霞;E-mail: hkdzlx@126.com
摘 要:
本文对苘麻种子吸水率进行测定, 对其种皮与种仁中萌发抑制物质活性进行分析, 并采用GA3对苘麻进行促萌试验, 探索了苘麻种子的休眠机理与打破休眠的方法。结果表明: 造成苘麻种子休眠的原因包括种皮障碍以及种皮与种仁中存在的抑制物质, 其中种皮透水性差与种子中存在内源抑制物质是限制苘麻种子萌发的主要原因, 其休眠属于综合休眠。在60°C温水中浸30 min后, 再用GA3处理可以促进苘麻种子的萌发; 其中, 采用400 mg•kg-1 GA3溶液处理种子24 h可以有效打破苘麻种子休眠, 较好地提高苘麻种子的萌发能力。关键词:苘麻; 种子; 休眠机理; 内源抑制物; 破眠方法
收稿:2016-04-27 修定:2016-05-20
资助:河南省高等学校重点科研项目(15A180037和16A220005)、河南省科技攻关计划项目(162102110095)、河南科技大学高级别项目培育基金(2015GJB029)、河南科技大学青年基金项目(4024-13350066和4026-13350041)和河南科技大学博士科研启动基金项目(4024-13480054和4026-13480038)。
Corresponding author: ZHANG Li-Xia; E-mail: hkdzlx@126.com
Abstract:
In this experiment, Abutilon theophrasti seeds were used as experimental material, the dormancy mechanism and dormancy breaking approaches of A. theophrasti seeds were investingated by water absorption rate test and biologlogical activity tests of crude extracts, and the feasible measures of improving germination rate were found by treating A. theophrasti seeds with gibberellin germination. These results showed that: the reasons of seed dormancy mechanism could be attributed to water absorption barrier caused by seed testa, germination endogenous inhibiting substance existing in testa and kernel, which were the primary limit factor. In conclusion, A. theophrasti seeds were featured multiple dormancies, the treatment (pre-soaking with 60°C water for 30 min, then soaked in 400 mg•kg-1 GA3 for 24 h) could break its dormancy effectly.Key words: Abutilon theophrasti; seeds; dormancy mechanism; endogenous inhibitor; dormancy breaking approaches
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